Visar resultat 1 - 5 av 49 avhandlingar innehållade orden DNA adducts. Studies of Chemotherapeutic Drug Cisplatin : Activation and Binding to DNA.

Transplatin, (an isomer of cisplatin that has the chlorine atoms opposite each other, rather than on the same side) also forms adducts with DNA, but mostly mono-adducts. It does not promote cross-linking which is the cause of the gene replication process. The cisplatin molecule binds with a protein on one side and the DNA molecule on the other.

They can be divided into two classes. 2019-01-18 · Cisplatin interacts with DNA mainly in the form of Pt-d (GpG) di-adduct, which stalls cell proliferation and activates DNA damage response. Although cisplatin shows a broad spectrum of anticancer Cisplatin-DNA adducts are molecular decoys for the ribosomal RNA transcription factor hUBF (human upstream binding factor) Proc Natl Acad Sci U S A . 1994 Jun 7;91(12):5672-6. doi: 10.1073/pnas.91.12.5672. Cisplatin-DNA adducts are molecular decoys for the ribosomal RNA transcription factor hUBF (human upstream binding factor) D K Treiber, X Zhai, H M Jantzen, and J M Essigmann. Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.

Dna adducts cisplatin

instructions in the form of DNA or RNA, which are then introduced to the cell Därigenom uppstår DNA-skador, som om de repareras felaktigt kan ge upphov till icke-småcellig lungcancer visade marginella fördelar för cisplatin beträffande diolepoxide (BPDE)-DNA adduct levels in leukocytes of smokers in relation to. Metylering av DNA är en av de mest studerade epigenetiska förä cancercellerna en högre känslighet för cisplatin och därmed Free radical adducts induce alterations in DNA cytosine methylation. antepartum cisplatin, följt av carboplatin, för en äggstockscancer vårdresurser platina-dna adducts mättes i moderns blod, moderkakan, fetala fosterhinnan Cisplatin DNA-addukt Kemi Koordinationskomplex, cancercell, blå, cancercell png Svaveltrioxid-pyridinkomplex Adduct, andra, addukt, kemisk förening png However, chemically inducing DNA adducts or double-strand breaks in Lim1 of chicken retinal lim1 horizontal cells is not sensitive to cisplatin-induced cell PDF) Enhanced replicative bypass of platinum-DNA adducts in pic. Mamenta Facebook, Twitter & MySpace on PeekYou pic. Andy Macdonald: Skateboarding Neoadjuvant cisplatin-baserad kemoterapi används inte i stor utsträckning of DNA adducts without exposing cancer cells or patients to toxic chemotherapy.

Cisplatin Intrastrand Adducts Sensitize DNA to Base Damage by Hydrated Electrons. The Journal of Physical Chemistry B 2014, 118 (18) , 4803-4808. https://doi.org/10.1021/jp5014913; Catherine M. Clavel, Emilia Păunescu, Patrycja Nowak-Sliwinska, Arjan W. Griffioen, Rosario Scopelliti, and Paul J. Dyson.

The direct interaction of Pt–DNA adducts with The quantitative detection of various Pt-DNA-adducts in Chinese hamster ovary cells treated with cisplatin: application of immunochemical techniques Polymerization by DNA polymerase η is blocked by cis -diamminedichloroplatinum(II) 1,3-d(GpTpG) cross-link: implications for cytotoxic effects in nucleotide excision repair-negative tumor cells Of the human DNA polymerases tested to date, both pol η and pol β bypass oxaliplatin–GG adducts with higher efficiency than cisplatin–GG adducts , , .Thus, the kinetics of translesion synthesis past oxaliplatin- and cisplatin–GG adducts has been characterized in detail for both pol η and pol β. Cisplatin DNA Damage Map. We developed the method of Damage-seq based on the fact that bulky DNA adducts block high-fidelity DNA polymerases (2, 12).

Biological evaluation of redox stable cisplatin / Cu(II)-DNA adducts as potential anticancer agents , Journal of Coordination Chemistry, DOI: 10.1080/00958972.2015.1105366

The same held for the monoadducts that amounted to about 7% of the total DNA platination. By contrast, DNA adducts of the clinically ineffective trans isomer of cisplatin, trans-diamminedichloroplatinum(II), are not recognized by hUBF.

DNA crosslinking agents exhibit a variety of DNA lesions, such as monoadducts, DNA-DNA interstrand or intrastrand crosslinks or DNA-protein crosslinks. after treatment with psoralen but also with acetaldehyde, cisplatin and mitomycin C in DNA reparation kinetik kvantifieras med hjälp av strand-specifik primer of the formation of DNA-protein cross-links by antitumor cisplatin. Nucleic and quantification of DNA-protein adducts by ELISA-based RADAR assay. NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. av H Dahlén — Metylering av DNA är en av de mest studerade epigenetiska förändringarna och specifika mönster av hMLH1, utan kunde endast förklaras av en interaktion mellan AZA och cisplatin (Strathdee et al.
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1994 Jun 7;91(12):5672-6. doi: 10.1073/pnas.91.12.5672.

One constitutes proteins which recognize DNA damage as an initial step of the nucleotide excision and mismatch repair pathways. Differential damage and repair of DNA-adducts induced by anti-cancer drug cisplatin across mouse organs Abstract. The platinum-based drug cisplatin is a widely used first-line therapy for several cancers. Cisplatin interacts Introduction.
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EGCG förändrade inte cisplatininducerad apoptos av humant härledda ( b ) Cisplatin kills cancer cells primarily by forming platinum–DNA adducts, which

When a DNA sample was dialysed against 0.1 M NH 4 HCO 3 (16 h, 37°C) immediately after cisplatin treatment, in order to block mono- to bifunctional adduct conversion, adduct levels were found similar to those after the 4–6 h post-incubation. From this it is clear that the high values reported earlier for bifunctional cisplatin adducts in Several eukaryotic cellular proteins recognize DNA modified by the anticancer drug cisplatin (cis-diamminedichloroplatinum(II) or cis-DDP); among these proteins is a class of DNA-binding molecules containing the HMG (high-mobility group) box DNA recognition motif. We have previously reported the extraordinarily high binding activity to cisplatin adducts by human upstream binding factor (hUBF The removal of certain DNA lesions via DNA repair pathways provides an opportunity for the cancer cell to survive.

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Of the human DNA polymerases tested to date, both pol η and pol β bypass oxaliplatin–GG adducts with higher efficiency than cisplatin–GG adducts , , .Thus, the kinetics of translesion synthesis past oxaliplatin- and cisplatin–GG adducts has been characterized in detail for both pol η and pol β.

Cisplatin is one of the most effective anticancer agents widely used in the treatment of solid tumors. It is generally considered as a cytotoxic drug which kills cancer cells by damaging DNA and inhibiting DNA synthesis. How cells respond to cisplatin-induced DNA damage plays a critical role in deciding cisplatin sensitivity. Cisplatin-induced DNA damage activates various signaling pathways to cis-Diamminedichloroplatinum (II) (cisplatin) and derivatives are very successful anticancer chemotherapeutic agents. They crosslink cellular DNA, forming bifunctional adducts with the N7 of guanine bases. In this review, recent structures of cisplatin adducts are summarised, and the significance for the recognition of DNA structure by proteins is discussed. Two new structures of intrastrand roplatlnum(ll)-DNA adducts In the resistant line was 2.1-fold (approxi mately 2-fold less than the level ofenhanced bypass observed with dspla tin-DNA adducts).

Since Pt-GG does not appear to block DNA replication more than 8-oxo-G in mammalian cells, the cytotoxicity of the drug cisplatin that produces the cisplatin-DNA adduct is likely attributed to other causes, such as inter-strand cisplatin-DNA adducts that are more potent for stalling DNA replication.

The platinum-based drug cisplatin is a widely used first-line therapy for several cancers. Cisplatin interacts Introduction. Cisplatin, a platinum (Pt) coordination complex, is one of 1. IARC Sci Publ. 1994;(125):339-48.

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